The Person-Centered Approach

The Person-Centered Approach Free Online Research Papers Over the past 50 years, counselors have begun to accept different approaches to the therapeutic process then those used previously. It was because of the initial thoughts put forth by a man named Carl Rogers, and his belief that there is more to the therapy process then the authoritarian approach that was being used at the time. Over the course of 4 phases in Roger’s development came about the concept of the Person-Centered approach. Adopted by counselors in the 60’s and 70’s as a part of the â€Å"Third Force†, along with existential and gestalt therapy, as an alternative to psychoanalytic and behavioral approaches (Corey, 2005). Person-Centered Therapy was never presented as a completed work, rather one of which Rogers hoped other would merely use as principles willing to be evolved. These principles were looked at by Rogers as required to gain a good therapist – client relationship which was a prime determinant of the outcome of the therapeutic process. These principles best outlined by Roger’s (1956) as, Two person’s in psychological contact. The first, whom we shall term the client, is in a state of incongruence, being vulnerable, or anxious. The second person, whom we shall term the therapist, is congruent or integrated in the relationship. The therapist experience unconditional positive regard for the client. The therapist experiences an empathetic understanding of the client’s internal frame of reference and endeavors to communicate this experience to the client. The future has brought about much need for the evolution of this theory, but this paper is meant to focus on these principles set forth by Rogers. Overtime the clients and problems may have changed, but by keeping these principles in mind, the approach is still the same. It was with a humanistic philosophy that Rogers created the person – centered approach, and with his principles rooted deeply in humanistic values, it is necessary to have a good understanding of how they came to be. The humanistic philosophy is similar yet much different from that of existentialism, of which takes the position that we are faced with the anxiety of choosing to create an identity in a world that lacks intrinsic meaning (Corey, 2005). The humanistic philosophy takes a somewhat less anxiety evoking position that each of us has a natural potential that we can actualize and through which we can find meaning (Corey, 2005). It was within this concept that Rogers developed his theory over the years. At the time there was only an authoritarian approach to counseling, of which Roger’s disagreed with, which started his theory of a non directive climate in the 1940’s. It was within this approach that Roger’s looked to stop the amount of input a therapist contributed to the counseling process; instead therapists concentrated on the clients verbal and non verbal communications. This approach over time evolved into a form of therapy referred to as client – centered. With this form Roger’s led away from the focus on the non directive approach and started focusing primarily on the client. With the client in mind, the next period of evolution that Roger’s encountered is where he defined his principles of which he wrote about in his paper, â€Å"The Necessary and Sufficient Conditions of Therapeutic Personality Change.† It is in this paper that Roger’s presented his theory of psychotherapy as a set of therapist â€Å"Core Attitudes† (Prouty, 1994). It is with these â€Å"Core Attitudes† that Roger’s defined the aim of the therapeutic process, â€Å"the aim of therapy is not merely to solve problems, rather; it is to assist clients in their growth process, so that the y can better cope with the problems they are now facing and with future problems.† (Corey, 2005). It was in the principles of; congruence, unconditional positive regard, and accurate empathetic understanding, that we find a way to promote a growth producing climate. Congruence Congruence or genuineness, involves letting the other person know â€Å"where you are† emotionally (Rogers, 1980). This can be either positive or negative, and the therapist must express their feelings to be as genuine as possible. The reason that the therapist must express all feelings toward their client is to avoid the temptations to present a faà §ade or hide behind a mask of professionalism or to assume a confessional professional attitude (Germain, 1993). The reason that it is so important for the therapist to become congruent with their client is to create a trusting relationship of which the client can let the therapist into their lives. Rogers gradually came to look at the therapist’s congruence as a crucial factor in establishing trust, and came to emphasize the idea of acceptance and empathy only being effective when they are perceived as genuine (Germain, 1993). In the views of Rogers it is essential to be living together in a climate of realness (Rog ers, 1980). This realness can only be assumed when the therapist can be completely open with their client as to how they feel about their presenting problem as well as the client’s way of life as a whole. This action of being completely open does not always have to be viewed in a good light by the client, but as long as the client knows that the therapist is being completely open with them, then the process can continue. This way of being is described here by Rogers in 1961, â€Å"The term ‘congruent’ is one I have used to describe the way I would like to be. By this I mean that whatever I am feeling or attitude I am experiencing would be matched by my awareness of that attitude.† It is within this self awareness that makes acceptance possible because there can be no openness to the clients experience if there is no openness to one’s own experience (Germain, 1993). Unconditional Positive Regard Once congruence between the therapist and the client is achieved, it is time to move on to the next principle that Rogers distinguished. Unconditional positive regard is exactly that, no matter what the client is feeling, the therapist must show a positive approval of how they are feeling. This is due to the true importance of unconditional positive regard and its power to build up or restore the client’s unconditional positive self regard (Iberg, 1996). It is Rogers’s theory that most of the clients that a therapist will see, have merely been looked over and never really made feel like they are accepted or cared for. It is within this principal that Rogers attempts to show an acceptance and unconditional caring for the client as â€Å"who they are† rather then who they are expected to be or as Rogers states it as â€Å"a caring for the client, but not in a possessive way or in such a way as simply to satisfy the therapists own needs† (Rogers, 1992). The therapist must make the client feel that they are able to convey their thoughts without fear of rejection or loss of acceptance by the therapist. It is the therapist job to make sure that their basis for caring for their client is not because of their need to be accepted themselves. This might hinder the experience that the client needs to go through as much as if the therapist had no respect or a dislike of their client. That is why congruence needs to be developed before the therapeutic process can begin, without a genuine approach to caring for the client the whole process will not be successful. Accurate Empathetic Understanding After the first two steps of Rogers’s theory have been completed then the therapist can continue on to an accurate empathetic understanding. This principle is based off the idea of empathy as Rogers defines â€Å"to sense the clients private world as if it were your own, but without ever losing the â€Å"as if† quality† (Rogers, 1992). That is the key to being an empathetic therapist, to never lose the â€Å"as if† mentality, to sense the clients feelings without ever getting caught up in them as if they were your own. The Merriam Webster Dictionary states that Empathy â€Å"is the action of understanding, being aware of, or being sensitive to.† This is exactly what Rogers meant by putting it into the principles of what a good therapist needs to do during therapy, to be able to understand and accurately be aware of the problems that the client is conveying. This is indicated in research done by Fieldler in which items such as the following pla ced high in the description of a relationship created by an experienced therapist. The therapist is well able to understand the patient’s feelings; the therapist is never in any doubt about what the patient means; the therapist’s remarks fit in just right with the patients mood and content; the therapists tone of voice conveys the complete ability to share the patients feelings. (Rogers, 1992) With all of these principals working together to create a valuable client-therapist relationship, an accurate empathetic understanding is one of the most important. The client must first be able to trust the therapist, and then feel that the therapist actually cares about what is being said, but without an accurate understanding of what is being said then the client would not feel as though the therapist is actually listening. Summary It was my intent with this paper to explain the principals of the person centered approach created by Carl Rogers. The reason that I feel that this approach is meaningful is that I have always felt that anyone has the ability to figure out their own problems. In life it is easy for people to get knocked off their horse, in a matter of speaking, but it is how we get back on that horse and continue to live is how living life is possible. Sometimes I feel as though it is hard to get up off the ground by yourself but the ability is still inside of you. That is why when I first heard about the person-centered approach, I was so intrigued by the elements that made up this theory. These principles that Rogers has developed, help to create an environment at which clients are able to hash out their problems in a self directed way. The therapist is merely a listener, there to make the client feel that what they are feeling is ordinary. Even though they may long for an authoritarian â⠂¬Å"answer†, the therapist does not give one yet just responds with points that the client has already made. Even though Rogers created this theory in a different time and age, when, if presented with some of the problems that therapist face today, it may have been harder to meet the criteria for his principles. I still feel as though these principals can be effective in any therapeutic situation. Today many person-centered therapists are focused on the family, however it has been found to be effective in many areas of therapy. I personally can see it being very effective in cases of depression, where a client is so low in positive self regard that they need to feel that someone else can actually care for them enough to listen. In research done by Greenberg and Watson (1998), of which compared client centered therapy and an experimental treatment using the principals of client centered therapy and its effect on depression, found that both had an effect on the therapy’s outcome. It was the experimental conditional along with the principals based of Rogers’s theory that ha d superior effects on the overall levels of self esteem and reduction of interpersonal problems. This research shows that although alone, these principals may not always be the exact answer, paired with other ideas it can have an impact on the client’s life. This paper is to show that these principals are in fact pillars or fundamentals of any and all problems that may be presented to a therapist. These are just attitudes that if followed correctly can bring about an atmosphere for change in a clients life. As Rogers states it, â€Å"people are essentially trustworthy, that they have a vast potential for understanding themselves and resolving their own problems without direct intervention on the therapists part, and that they are capable of self directed growth if they are involved in a specific kind of therapeutic relationship† (Corey, 2005). Corey, Gerald. (2005) Theory and Practice of Counseling Psychotherapy. Thomson Books; Belmont, CA. Greenberg L., Watson J, (1998) Experiential Therapy of Depression: Differential Effects of Client-Centered Relationship Conditions and Process Experiential Interventions. The Journal for Psychotherapy Research. Vol. 8, Number 2, pp. 210- 224 Iberg, J.R. (1996) Finding the body’s next step: Ingredients and hindrances. The Journal for focusing and experiential therapy, vol 15, 13-42. Lietaer, Germain (1993), Beyond Carl Rogers, Constable, London Prouty, Gary, (1994) Theoretical evolutions in person-centered/ experiential therapy. Applications to schizophrenic and retarded psychosis. Praeger Publishers, Westport, CT Rogers, Carl (1980) A way of being. Rogers, Carl (1992) The necessary and sufficient conditions of therapeutic personality change. Journal of Consulting and Clinical Psychology, Dec 1992, vol. 60, No. 6 pp. 827-832. Research Papers on The Person-Centered ApproachThree Concepts of PsychodynamicThe Project Managment Office SystemOpen Architechture a white paperIncorporating Risk and Uncertainty Factor in CapitalGenetic EngineeringEffects of Television Violence on ChildrenRelationship between Media Coverage and Social andComparison: Letter from Birmingham and CritoBionic Assembly System: A New Concept of SelfThe Relationship Between Delinquency and Drug Use

The Person-Centered Approach

The Person-Centered Approach Free Online Research Papers Over the past 50 years, counselors have begun to accept different approaches to the therapeutic process then those used previously. It was because of the initial thoughts put forth by a man named Carl Rogers, and his belief that there is more to the therapy process then the authoritarian approach that was being used at the time. Over the course of 4 phases in Roger’s development came about the concept of the Person-Centered approach. Adopted by counselors in the 60’s and 70’s as a part of the â€Å"Third Force†, along with existential and gestalt therapy, as an alternative to psychoanalytic and behavioral approaches (Corey, 2005). Person-Centered Therapy was never presented as a completed work, rather one of which Rogers hoped other would merely use as principles willing to be evolved. These principles were looked at by Rogers as required to gain a good therapist – client relationship which was a prime determinant of the outcome of the therapeutic process. These principles best outlined by Roger’s (1956) as, Two person’s in psychological contact. The first, whom we shall term the client, is in a state of incongruence, being vulnerable, or anxious. The second person, whom we shall term the therapist, is congruent or integrated in the relationship. The therapist experience unconditional positive regard for the client. The therapist experiences an empathetic understanding of the client’s internal frame of reference and endeavors to communicate this experience to the client. The future has brought about much need for the evolution of this theory, but this paper is meant to focus on these principles set forth by Rogers. Overtime the clients and problems may have changed, but by keeping these principles in mind, the approach is still the same. It was with a humanistic philosophy that Rogers created the person – centered approach, and with his principles rooted deeply in humanistic values, it is necessary to have a good understanding of how they came to be. The humanistic philosophy is similar yet much different from that of existentialism, of which takes the position that we are faced with the anxiety of choosing to create an identity in a world that lacks intrinsic meaning (Corey, 2005). The humanistic philosophy takes a somewhat less anxiety evoking position that each of us has a natural potential that we can actualize and through which we can find meaning (Corey, 2005). It was within this concept that Rogers developed his theory over the years. At the time there was only an authoritarian approach to counseling, of which Roger’s disagreed with, which started his theory of a non directive climate in the 1940’s. It was within this approach that Roger’s looked to stop the amount of input a therapist contributed to the counseling process; instead therapists concentrated on the clients verbal and non verbal communications. This approach over time evolved into a form of therapy referred to as client – centered. With this form Roger’s led away from the focus on the non directive approach and started focusing primarily on the client. With the client in mind, the next period of evolution that Roger’s encountered is where he defined his principles of which he wrote about in his paper, â€Å"The Necessary and Sufficient Conditions of Therapeutic Personality Change.† It is in this paper that Roger’s presented his theory of psychotherapy as a set of therapist â€Å"Core Attitudes† (Prouty, 1994). It is with these â€Å"Core Attitudes† that Roger’s defined the aim of the therapeutic process, â€Å"the aim of therapy is not merely to solve problems, rather; it is to assist clients in their growth process, so that the y can better cope with the problems they are now facing and with future problems.† (Corey, 2005). It was in the principles of; congruence, unconditional positive regard, and accurate empathetic understanding, that we find a way to promote a growth producing climate. Congruence Congruence or genuineness, involves letting the other person know â€Å"where you are† emotionally (Rogers, 1980). This can be either positive or negative, and the therapist must express their feelings to be as genuine as possible. The reason that the therapist must express all feelings toward their client is to avoid the temptations to present a faà §ade or hide behind a mask of professionalism or to assume a confessional professional attitude (Germain, 1993). The reason that it is so important for the therapist to become congruent with their client is to create a trusting relationship of which the client can let the therapist into their lives. Rogers gradually came to look at the therapist’s congruence as a crucial factor in establishing trust, and came to emphasize the idea of acceptance and empathy only being effective when they are perceived as genuine (Germain, 1993). In the views of Rogers it is essential to be living together in a climate of realness (Rog ers, 1980). This realness can only be assumed when the therapist can be completely open with their client as to how they feel about their presenting problem as well as the client’s way of life as a whole. This action of being completely open does not always have to be viewed in a good light by the client, but as long as the client knows that the therapist is being completely open with them, then the process can continue. This way of being is described here by Rogers in 1961, â€Å"The term ‘congruent’ is one I have used to describe the way I would like to be. By this I mean that whatever I am feeling or attitude I am experiencing would be matched by my awareness of that attitude.† It is within this self awareness that makes acceptance possible because there can be no openness to the clients experience if there is no openness to one’s own experience (Germain, 1993). Unconditional Positive Regard Once congruence between the therapist and the client is achieved, it is time to move on to the next principle that Rogers distinguished. Unconditional positive regard is exactly that, no matter what the client is feeling, the therapist must show a positive approval of how they are feeling. This is due to the true importance of unconditional positive regard and its power to build up or restore the client’s unconditional positive self regard (Iberg, 1996). It is Rogers’s theory that most of the clients that a therapist will see, have merely been looked over and never really made feel like they are accepted or cared for. It is within this principal that Rogers attempts to show an acceptance and unconditional caring for the client as â€Å"who they are† rather then who they are expected to be or as Rogers states it as â€Å"a caring for the client, but not in a possessive way or in such a way as simply to satisfy the therapists own needs† (Rogers, 1992). The therapist must make the client feel that they are able to convey their thoughts without fear of rejection or loss of acceptance by the therapist. It is the therapist job to make sure that their basis for caring for their client is not because of their need to be accepted themselves. This might hinder the experience that the client needs to go through as much as if the therapist had no respect or a dislike of their client. That is why congruence needs to be developed before the therapeutic process can begin, without a genuine approach to caring for the client the whole process will not be successful. Accurate Empathetic Understanding After the first two steps of Rogers’s theory have been completed then the therapist can continue on to an accurate empathetic understanding. This principle is based off the idea of empathy as Rogers defines â€Å"to sense the clients private world as if it were your own, but without ever losing the â€Å"as if† quality† (Rogers, 1992). That is the key to being an empathetic therapist, to never lose the â€Å"as if† mentality, to sense the clients feelings without ever getting caught up in them as if they were your own. The Merriam Webster Dictionary states that Empathy â€Å"is the action of understanding, being aware of, or being sensitive to.† This is exactly what Rogers meant by putting it into the principles of what a good therapist needs to do during therapy, to be able to understand and accurately be aware of the problems that the client is conveying. This is indicated in research done by Fieldler in which items such as the following pla ced high in the description of a relationship created by an experienced therapist. The therapist is well able to understand the patient’s feelings; the therapist is never in any doubt about what the patient means; the therapist’s remarks fit in just right with the patients mood and content; the therapists tone of voice conveys the complete ability to share the patients feelings. (Rogers, 1992) With all of these principals working together to create a valuable client-therapist relationship, an accurate empathetic understanding is one of the most important. The client must first be able to trust the therapist, and then feel that the therapist actually cares about what is being said, but without an accurate understanding of what is being said then the client would not feel as though the therapist is actually listening. Summary It was my intent with this paper to explain the principals of the person centered approach created by Carl Rogers. The reason that I feel that this approach is meaningful is that I have always felt that anyone has the ability to figure out their own problems. In life it is easy for people to get knocked off their horse, in a matter of speaking, but it is how we get back on that horse and continue to live is how living life is possible. Sometimes I feel as though it is hard to get up off the ground by yourself but the ability is still inside of you. That is why when I first heard about the person-centered approach, I was so intrigued by the elements that made up this theory. These principles that Rogers has developed, help to create an environment at which clients are able to hash out their problems in a self directed way. The therapist is merely a listener, there to make the client feel that what they are feeling is ordinary. Even though they may long for an authoritarian â⠂¬Å"answer†, the therapist does not give one yet just responds with points that the client has already made. Even though Rogers created this theory in a different time and age, when, if presented with some of the problems that therapist face today, it may have been harder to meet the criteria for his principles. I still feel as though these principals can be effective in any therapeutic situation. Today many person-centered therapists are focused on the family, however it has been found to be effective in many areas of therapy. I personally can see it being very effective in cases of depression, where a client is so low in positive self regard that they need to feel that someone else can actually care for them enough to listen. In research done by Greenberg and Watson (1998), of which compared client centered therapy and an experimental treatment using the principals of client centered therapy and its effect on depression, found that both had an effect on the therapy’s outcome. It was the experimental conditional along with the principals based of Rogers’s theory that ha d superior effects on the overall levels of self esteem and reduction of interpersonal problems. This research shows that although alone, these principals may not always be the exact answer, paired with other ideas it can have an impact on the client’s life. This paper is to show that these principals are in fact pillars or fundamentals of any and all problems that may be presented to a therapist. These are just attitudes that if followed correctly can bring about an atmosphere for change in a clients life. As Rogers states it, â€Å"people are essentially trustworthy, that they have a vast potential for understanding themselves and resolving their own problems without direct intervention on the therapists part, and that they are capable of self directed growth if they are involved in a specific kind of therapeutic relationship† (Corey, 2005). Corey, Gerald. (2005) Theory and Practice of Counseling Psychotherapy. Thomson Books; Belmont, CA. Greenberg L., Watson J, (1998) Experiential Therapy of Depression: Differential Effects of Client-Centered Relationship Conditions and Process Experiential Interventions. The Journal for Psychotherapy Research. Vol. 8, Number 2, pp. 210- 224 Iberg, J.R. (1996) Finding the body’s next step: Ingredients and hindrances. The Journal for focusing and experiential therapy, vol 15, 13-42. Lietaer, Germain (1993), Beyond Carl Rogers, Constable, London Prouty, Gary, (1994) Theoretical evolutions in person-centered/ experiential therapy. Applications to schizophrenic and retarded psychosis. Praeger Publishers, Westport, CT Rogers, Carl (1980) A way of being. Rogers, Carl (1992) The necessary and sufficient conditions of therapeutic personality change. Journal of Consulting and Clinical Psychology, Dec 1992, vol. 60, No. 6 pp. 827-832. Research Papers on The Person-Centered ApproachThree Concepts of PsychodynamicThe Project Managment Office SystemOpen Architechture a white paperIncorporating Risk and Uncertainty Factor in CapitalGenetic EngineeringEffects of Television Violence on ChildrenRelationship between Media Coverage and Social andComparison: Letter from Birmingham and CritoBionic Assembly System: A New Concept of SelfThe Relationship Between Delinquency and Drug Use

The Person-Centered Approach

The Person-Centered Approach Free Online Research Papers Over the past 50 years, counselors have begun to accept different approaches to the therapeutic process then those used previously. It was because of the initial thoughts put forth by a man named Carl Rogers, and his belief that there is more to the therapy process then the authoritarian approach that was being used at the time. Over the course of 4 phases in Roger’s development came about the concept of the Person-Centered approach. Adopted by counselors in the 60’s and 70’s as a part of the â€Å"Third Force†, along with existential and gestalt therapy, as an alternative to psychoanalytic and behavioral approaches (Corey, 2005). Person-Centered Therapy was never presented as a completed work, rather one of which Rogers hoped other would merely use as principles willing to be evolved. These principles were looked at by Rogers as required to gain a good therapist – client relationship which was a prime determinant of the outcome of the therapeutic process. These principles best outlined by Roger’s (1956) as, Two person’s in psychological contact. The first, whom we shall term the client, is in a state of incongruence, being vulnerable, or anxious. The second person, whom we shall term the therapist, is congruent or integrated in the relationship. The therapist experience unconditional positive regard for the client. The therapist experiences an empathetic understanding of the client’s internal frame of reference and endeavors to communicate this experience to the client. The future has brought about much need for the evolution of this theory, but this paper is meant to focus on these principles set forth by Rogers. Overtime the clients and problems may have changed, but by keeping these principles in mind, the approach is still the same. It was with a humanistic philosophy that Rogers created the person – centered approach, and with his principles rooted deeply in humanistic values, it is necessary to have a good understanding of how they came to be. The humanistic philosophy is similar yet much different from that of existentialism, of which takes the position that we are faced with the anxiety of choosing to create an identity in a world that lacks intrinsic meaning (Corey, 2005). The humanistic philosophy takes a somewhat less anxiety evoking position that each of us has a natural potential that we can actualize and through which we can find meaning (Corey, 2005). It was within this concept that Rogers developed his theory over the years. At the time there was only an authoritarian approach to counseling, of which Roger’s disagreed with, which started his theory of a non directive climate in the 1940’s. It was within this approach that Roger’s looked to stop the amount of input a therapist contributed to the counseling process; instead therapists concentrated on the clients verbal and non verbal communications. This approach over time evolved into a form of therapy referred to as client – centered. With this form Roger’s led away from the focus on the non directive approach and started focusing primarily on the client. With the client in mind, the next period of evolution that Roger’s encountered is where he defined his principles of which he wrote about in his paper, â€Å"The Necessary and Sufficient Conditions of Therapeutic Personality Change.† It is in this paper that Roger’s presented his theory of psychotherapy as a set of therapist â€Å"Core Attitudes† (Prouty, 1994). It is with these â€Å"Core Attitudes† that Roger’s defined the aim of the therapeutic process, â€Å"the aim of therapy is not merely to solve problems, rather; it is to assist clients in their growth process, so that the y can better cope with the problems they are now facing and with future problems.† (Corey, 2005). It was in the principles of; congruence, unconditional positive regard, and accurate empathetic understanding, that we find a way to promote a growth producing climate. Congruence Congruence or genuineness, involves letting the other person know â€Å"where you are† emotionally (Rogers, 1980). This can be either positive or negative, and the therapist must express their feelings to be as genuine as possible. The reason that the therapist must express all feelings toward their client is to avoid the temptations to present a faà §ade or hide behind a mask of professionalism or to assume a confessional professional attitude (Germain, 1993). The reason that it is so important for the therapist to become congruent with their client is to create a trusting relationship of which the client can let the therapist into their lives. Rogers gradually came to look at the therapist’s congruence as a crucial factor in establishing trust, and came to emphasize the idea of acceptance and empathy only being effective when they are perceived as genuine (Germain, 1993). In the views of Rogers it is essential to be living together in a climate of realness (Rog ers, 1980). This realness can only be assumed when the therapist can be completely open with their client as to how they feel about their presenting problem as well as the client’s way of life as a whole. This action of being completely open does not always have to be viewed in a good light by the client, but as long as the client knows that the therapist is being completely open with them, then the process can continue. This way of being is described here by Rogers in 1961, â€Å"The term ‘congruent’ is one I have used to describe the way I would like to be. By this I mean that whatever I am feeling or attitude I am experiencing would be matched by my awareness of that attitude.† It is within this self awareness that makes acceptance possible because there can be no openness to the clients experience if there is no openness to one’s own experience (Germain, 1993). Unconditional Positive Regard Once congruence between the therapist and the client is achieved, it is time to move on to the next principle that Rogers distinguished. Unconditional positive regard is exactly that, no matter what the client is feeling, the therapist must show a positive approval of how they are feeling. This is due to the true importance of unconditional positive regard and its power to build up or restore the client’s unconditional positive self regard (Iberg, 1996). It is Rogers’s theory that most of the clients that a therapist will see, have merely been looked over and never really made feel like they are accepted or cared for. It is within this principal that Rogers attempts to show an acceptance and unconditional caring for the client as â€Å"who they are† rather then who they are expected to be or as Rogers states it as â€Å"a caring for the client, but not in a possessive way or in such a way as simply to satisfy the therapists own needs† (Rogers, 1992). The therapist must make the client feel that they are able to convey their thoughts without fear of rejection or loss of acceptance by the therapist. It is the therapist job to make sure that their basis for caring for their client is not because of their need to be accepted themselves. This might hinder the experience that the client needs to go through as much as if the therapist had no respect or a dislike of their client. That is why congruence needs to be developed before the therapeutic process can begin, without a genuine approach to caring for the client the whole process will not be successful. Accurate Empathetic Understanding After the first two steps of Rogers’s theory have been completed then the therapist can continue on to an accurate empathetic understanding. This principle is based off the idea of empathy as Rogers defines â€Å"to sense the clients private world as if it were your own, but without ever losing the â€Å"as if† quality† (Rogers, 1992). That is the key to being an empathetic therapist, to never lose the â€Å"as if† mentality, to sense the clients feelings without ever getting caught up in them as if they were your own. The Merriam Webster Dictionary states that Empathy â€Å"is the action of understanding, being aware of, or being sensitive to.† This is exactly what Rogers meant by putting it into the principles of what a good therapist needs to do during therapy, to be able to understand and accurately be aware of the problems that the client is conveying. This is indicated in research done by Fieldler in which items such as the following pla ced high in the description of a relationship created by an experienced therapist. The therapist is well able to understand the patient’s feelings; the therapist is never in any doubt about what the patient means; the therapist’s remarks fit in just right with the patients mood and content; the therapists tone of voice conveys the complete ability to share the patients feelings. (Rogers, 1992) With all of these principals working together to create a valuable client-therapist relationship, an accurate empathetic understanding is one of the most important. The client must first be able to trust the therapist, and then feel that the therapist actually cares about what is being said, but without an accurate understanding of what is being said then the client would not feel as though the therapist is actually listening. Summary It was my intent with this paper to explain the principals of the person centered approach created by Carl Rogers. The reason that I feel that this approach is meaningful is that I have always felt that anyone has the ability to figure out their own problems. In life it is easy for people to get knocked off their horse, in a matter of speaking, but it is how we get back on that horse and continue to live is how living life is possible. Sometimes I feel as though it is hard to get up off the ground by yourself but the ability is still inside of you. That is why when I first heard about the person-centered approach, I was so intrigued by the elements that made up this theory. These principles that Rogers has developed, help to create an environment at which clients are able to hash out their problems in a self directed way. The therapist is merely a listener, there to make the client feel that what they are feeling is ordinary. Even though they may long for an authoritarian â⠂¬Å"answer†, the therapist does not give one yet just responds with points that the client has already made. Even though Rogers created this theory in a different time and age, when, if presented with some of the problems that therapist face today, it may have been harder to meet the criteria for his principles. I still feel as though these principals can be effective in any therapeutic situation. Today many person-centered therapists are focused on the family, however it has been found to be effective in many areas of therapy. I personally can see it being very effective in cases of depression, where a client is so low in positive self regard that they need to feel that someone else can actually care for them enough to listen. In research done by Greenberg and Watson (1998), of which compared client centered therapy and an experimental treatment using the principals of client centered therapy and its effect on depression, found that both had an effect on the therapy’s outcome. It was the experimental conditional along with the principals based of Rogers’s theory that ha d superior effects on the overall levels of self esteem and reduction of interpersonal problems. This research shows that although alone, these principals may not always be the exact answer, paired with other ideas it can have an impact on the client’s life. This paper is to show that these principals are in fact pillars or fundamentals of any and all problems that may be presented to a therapist. These are just attitudes that if followed correctly can bring about an atmosphere for change in a clients life. As Rogers states it, â€Å"people are essentially trustworthy, that they have a vast potential for understanding themselves and resolving their own problems without direct intervention on the therapists part, and that they are capable of self directed growth if they are involved in a specific kind of therapeutic relationship† (Corey, 2005). Corey, Gerald. (2005) Theory and Practice of Counseling Psychotherapy. Thomson Books; Belmont, CA. Greenberg L., Watson J, (1998) Experiential Therapy of Depression: Differential Effects of Client-Centered Relationship Conditions and Process Experiential Interventions. The Journal for Psychotherapy Research. Vol. 8, Number 2, pp. 210- 224 Iberg, J.R. (1996) Finding the body’s next step: Ingredients and hindrances. The Journal for focusing and experiential therapy, vol 15, 13-42. Lietaer, Germain (1993), Beyond Carl Rogers, Constable, London Prouty, Gary, (1994) Theoretical evolutions in person-centered/ experiential therapy. Applications to schizophrenic and retarded psychosis. Praeger Publishers, Westport, CT Rogers, Carl (1980) A way of being. Rogers, Carl (1992) The necessary and sufficient conditions of therapeutic personality change. Journal of Consulting and Clinical Psychology, Dec 1992, vol. 60, No. 6 pp. 827-832. Research Papers on The Person-Centered ApproachThree Concepts of PsychodynamicThe Project Managment Office SystemOpen Architechture a white paperIncorporating Risk and Uncertainty Factor in CapitalGenetic EngineeringEffects of Television Violence on ChildrenRelationship between Media Coverage and Social andComparison: Letter from Birmingham and CritoBionic Assembly System: A New Concept of SelfThe Relationship Between Delinquency and Drug Use

Using the Present Simple for ESL Students

Using the Present Simple for ESL Students The reading-comprehension passage below focuses on the present simple tense to describe habits and daily work routines. The present simple is typically one of the first verb tenses that new English students learn. It is used to describe an action that takes place on a regular basis. The present simple also can be used to express feelings, facts, opinion, and time-based events. The passage describes the daily routine and work habits of Tim, a typical worker in a central California city. Use the passage to help students better understand what is the present simple tense and how to use it. Before Reading the Passage Prepare students before they read the passage by explaining when to use the present simple tense and how to conjugate verbs in this tense. Explain that in English, you use the present simple to describe what you (or others) do every day. You also use verbs of frequency (such as always, sometimes, and usually) to indicate a habit. Ask students to tell you some things they do every day, such as setting the alarm before going to bed, waking up at a certain time each morning, eating breakfast, and traveling to work or school. Write their answers on the white board. Then explain that the present simple tense can be expressed in three ways: positive, negative, or as a question, for example: I eat lunch at noon.I never play tennis at noon.Does he walk to school every day? Tell students that theyll be reading a story about Tim, a worker who does a number of things regularly in getting ready for work, traveling to work, and performing his duties. Then read the story as a class, having students each read a sentence or two. Tims Story Tim works for a company in Sacramento. Hes a customer service representative. He gets up at 6 oclock a.m. each workday. He drives to work and begins his job at 8 oclock each morning. During the workday, Tim speaks to people on the telephone to help them with their banking problems. People telephone the bank to ask questions about their accounts. Tim doesnt give information about accounts until callers answer a few questions. Tim asks callers their birth date, the last four digits of their Social Security number, and their address. If a person gives incorrect information, Tim asks him to call back with the correct information. Tim is polite and friendly to everyone. He has lunch in a park next to his office. He returns home at 5 oclock in the evening. After work, he goes to the gym to work out. Tim has dinner at 7 oclock. Tim likes watching TV after dinner. He goes to bed at 11 oclock at night. Follow-Up Questions and Answers To extend the lesson, have students answer the following questions: What time does Time get up each workday? (6 oclock a.m.)What time does he begin his day at work each day? (8 a.m.)What are some of the duties Tim performs each day? (Tim verifies callers personal information. He answers questions from callers about their accounts. He is polite with each caller.)What time does Tim turn out the lights each night? (11 p.m.) Have students tell you a few more things Tim does each day as you complete your lesson on the present simple tense.

Types of Network Attacks Research Paper Example | Topics and Well Written Essays - 1000 words

Types of Network Attacks - Research Paper Example There is a variety of network attacks reported by researchers and information assurance professionals which keep targeting the government and private industry in a staggering number. It is a great concern of both the government and private industry to get rid of them by using the defense mechanisms identified by information assurance professionals in the past but significant research is still in continuation on this particular subject. It is reported that the number of attacks has doubled by two times since the introduction of World Wide Web due to which some say that the number of network attacks has become unprecedented now and the faster effective defense actions are taken against them, the better. The National Cyber Alert System for the US government and private sectors identified in recent past that all types of network attacks utilize security vulnerabilities (Yang, 1997) and nearly half of all the security threats from the Internet go unreported.It is increasingly important fo r the information assurance professionals and network administrators to acknowledge the nature of potential attacks on computer security so that it becomes easier afterwards to protect the network against such attacks. Spoofing is one such network attack which creates problems by causing a host or application to imitate the actions of another (The Linux Documentation Project, 2002). This is done by tracking IP addresses in network patches by the attacker who conventionally mimics another innocent host. After identifying the IP address by IP Address Spoofing, the attacker next proceeds to modifying or deleting important data (Microsoft TechNet, 2012). Following IP addresses means tracking or guessing TCP sequence number for example as in the case when a TCP connection from another host is tried to be mimicked by the attacker. This is why security vulnerabilities are created by the US-CERT to defend against such natured network attacks. The authenticity of various datagrams and comma nds is also advised to be verified by the teams responsible for detecting network attacks. This is identified as a very important step for protection against network attacks like spoofing. The attackers also try taking advantage of the element of predictability which is why it is advised to incorporate unpredictability into connection control systems so that TCP sequence numbers and myriad port addresses for example could not be tracked or mimicked through spoofing. Source addresses are also taken advantage of in spoofing due to which

Market Value and Change in Accounting Policy Essay

Market Value and Change in Accounting Policy - Essay Example Any organization in the market has to ensure that it chooses the correct accounting policy to attract more investors. The investors usually have an interest in studying the financial situation of the company to allow them to make an effective marketing decision. Insightfully, this means that the choice the company makes will affect the reflection and recording of financial statement to the investors. Consequently, the earning management by a company highly depends on the accounting policy that a firm uses in showing its financial position. Discussion The switch between FC and SE, as accounting methodologies, depending on the reasoning that one capitalizes while another expense the cost. Successful Efforts refers to the methodology where the unsuccessful exploration cost is expensed and usually integrated as part of the income statement. However, Full Cost involves capitalizing the unsuccessful exploration cost meaning that this cost is not part of the income statement in this situati on. The choosing of the two alternative methods relies on their effectiveness in achieving transparency related to the accounting information about oil and gas company’s earnings and cash flows. Based on the Successful Cost method, the objective of an oil and gas company is to produce oil or gas from its reserves hence the view that only the costs related to successful efforts are capitalized. On the other hand, the cost incurred is usually expensed, because successful results rely on the change in productive assets. Conversely, the FC method holds that the main objective of the oil and gas companies is to explore and develop oil and gas reserves. This implies that the costs incurred in the process of exploration and development should be capitalized followed by writing them off as the operation cycle continues. However, the regulatory approval from the Financial Accounting Standards Board (FASB), which looks over the establishment of the governing GAAP, required the oil and gas companies to adopt the SE method. In rejecting the change from FC to SE method, the users argued that this could substantially depress reported earnings and equity figures; and increase the volatility of earnings over time. This means that a change from FC to SE will reduce the capabilities of the firm to raise capital in the stock market thereby leading to vulnerability to competition. The increased volatility of earnings implicates a limitation in the ability of the firms to carry out new explorations in the industry. To support its view on the potential effect coming with adopting FASB Exposure draft, the journal presents statistics showing that 70 out of 109 FC firms would have their average earnings reduced by at least 5%; and 86% of these companies will also witness at least a 5% decrease in their owner’s equity (Lev, 1979, p. 487). Impact of the change on cash flow The shift in the accounting method, from FC to SE had no impact on the cash flow albeit the decrease in the market value. A no-effect theory as stated by HOLTHAUSEN, argues that there is no effect on the stock price associated with the change in accounting policy (Holthausen & Leftwich, 1998, p. 114). The accounting methods are just a facade for the accounting numbers, available for the investors. The author notes that the firms, adopting policy change can unravel the accounting numbers, without spending any dollar, implying that the choice of accounting methods do not affect the wealth of the company.     Ã‚  

Investigation of Tetracycline Antibacterial Activities

Investigation of Tetracycline Antibacterial Activities NOR FAEZAH BINTI ROSMIDI   Escherichia coli is a Gram-negative bacteria, which discovered and named after Theodor Escherichia and belong to Enterobacteriaceae family. E. coli is classified as same group as other bacterial such as Bordetella pertussis, Borrelia burgdoferi, Chlamydia trachomatis, Helicobacter pylori and many more species that are well known in medical world. E. coli infection can be found all over the world and more common in tropical country. This infection especially can be transmitted through diet because the infections are mainly due to contaminated food. Thus, this infection can be called as food-borned disease. An individual can get infected when consumed foods that are contaminated with dirt. However, not every individual that consumed contaminated food can get the infection. This is because, there are certain criteria to be fulfilled. Firstly, there must be source of infection, which means, E. coli must present in the food. Then, the individual is eating food contaminated with pathogen, as the main route of infection is through orofecal. Lastly, the bodys immune system cannot get rid of the pathogen, which eventually causing disease. This study is conducted to isolate, characterize and evaluate antimicrobial activities of E. coli in media and milk. A total of 3 sample is collected from the stock culture and labelled as EY1, E27 and E31. Isolation is made on the eosin methylene blue (EMB) agar and identified by using gram staining under microscope. To check for the antimicrobial activities, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time kill assay are done. Results reveals that the MIC value for E. coli is 128 and MBC value is 1024. Time-kill assay curve disclose that the reaction of tetracycline to the bacteria in media and milk slightly different but yet, able to achieve bactericidal effect of the drug. 1.0 INTRODUCTION 1.1 Introduction Mastitis, or inflammation of the mammary gland, is predominantly due to the effects of infection by bacterial pathogens, although mycotic or algal microbes play a role in some cases (Ronald J. Erskine, 2016b). There are many factors that influence the development of mastitis including microbial, host and environmental (Contreras Rodrà ­guez, 2011). Any opportunistic microbes that can invade tissue and causing infection can lead to mastitis. The pathogen that use this transmission mode are Staphylococcus aureus, Streptococcus agalactiae and Corynebacterium bovis. They may spread from cattle to another cattle through aerosol transmission and invade the udder causing bacteremia. There is other route of infection, which is through the environment of the cattle. The pathogens that associated with environmental reservoirs are Escherichia coli and Trueperella pyogenes (Ronald J. Erskine, 2016a)       Post-milking teat dipping, dry cow therapy, well-maintained milking equipment, and culling of cows with chronic mastitis have successfully controlled contagious mastitis bacteria such as Streptococcus agalactiae and Staphylococcus aureus (Makovec Ruegg, 2003). Milk samples collected from cows on Wisconsin dairy farms clearly demonstrated that environmental bacteria are the most common causes of clinical mastitis. (Pamela L. Ruegg, 2015) Once inside the udder, E. coli multiply rapidly, causing influx of the inflammatory cells. As neutrophils ingest and kill bacteria, endotoxin is released and along with other inflammatory mediators causes severe local inflammation. This inflammatory response is characterized by increased vascular permeability, changes in milk composition and damage to the mammary epithelial cells, which may result in the characteristic watery or serous milk secretion. The treatment for mastitis include administration of antimicrobial via the intramammary route or parentally (Gruet, Maincent, Berthelot, Kaltsatos, 2001). Unfortunately, despite the best possible antimicrobial treatments, failures of bacteriological cure are common, especially for S. aureus mastitis, and antimicrobial resistance (AMR) is considered one of the reasons for low cure rates (Candrasekaran et al., 2014). There are other reasons, which are the management and iatrogenic factors, drug factors, mastitis-causing organism factors and also mammary gland factor. However, the major reason is the insufficient contact of the antimicrobial with the mastitis-causing organisms at the site of infection is a major cause of mastitis treatment failure (Kiro R. Petrovski, 2007). Some bacteria such as S. aureus can localize inside host cells thus restrict the concentration of drug in the cells. E. coli can form biofilms, a condition where the microorganisms structured within an array of exopolysaccharides (EPS) and adhering to a living or inert surface with the function of protecting the microorganisms in stress environments (Costa, Espeschit, Pieri, Benjamin, Moreira, 2014). The sessile cells arranged in biofilms tend to be more resistant to antimicrobial therapy compared with plantonic free cells, due to the slow diffusion of the antimicrobial in the inner layers of the biofilm (Costa et al., 2014). Also, to be effective, drugs need to be free and not inhibited by other components. Some drug may have high affinity binding towards protein. Thus, some component in the milk, for example, casein, may bind to the antibiotic and reduce the free the drugs in the udder. In the case of S. aureus infections during lactation, it has been estimated that the bacteriological cure rate is only within 25% to 50% only (Kiro R. Petrovski, 2015). 1.2 Problem Statement Escherichia coli is one of the major cause of mastitis development. Based on the research done in Wisconsin dairy farm, 21% of the mastitis case result from the E. coli infection. Despite of all the treatment given, the therapy has not been successful. Hence, the aim of this study is to investigate the antibacterial activities of Tetracycline against E. coli from clinical mastitis in growth media and milk. 1.3 Research Questions This study is designed to answer either tetracycline can demonstrate equivalent antimicrobial efficacy against E.coli in growth medium and in milk? 1.4 Research Hypotheses This study is constructed to answer the hypothesis, either tetracycline demonstrates equivalent antimicrobial efficacy against E. coli in growth medium and milk. 1.5 Research Objectives This study is conducted to achieve a set of following objectives :- To determine minimum inhibitory concentrations (MIC) of Enrofloxacin and against the pathogenic E. coli To determine minimum bactericidal concentrations (MBC) of Enrofloxacin and against the pathogenic E. coli To perform time kill assay of Enrofloxacin against E. coli 2.0 LITERATURE REVIEW 2.1 Definition of mastitis Mastitis is swelling of the mammary gland that frequently caused by bacteria ingoing the teat canal and moving up to the udder and there are two types of mastitis, which are contagious mastitis and also environmental mastitis (Dairy Australia, 2007). Contagious or cow-associated mastitis generally related to the bacterial infection that causing disease, which are Staphylococcus aureus and Streptococcus agalactiae (Dairy Australia, 2007). The bacteria present in the udders or on teat skin and spread either by splashes of disease-ridden milk or sprays during stripping on milkers hands or teatcup liners and by cross flow of milk between teatcups (Dairy Australia, 2007). Meanwhile environmental mastitis occur when the bedding which used to house the cattle is contaminated (Ronald J. Erskine, 2016a). It is not only concern on the bedding of the cattle, the infected soil, manure, calving pads and water host bacteria can also causing this disease to developed (Dairy Australia, 2007). Mastitis come in two forms, which are subclinical and clinical, however, subclinical form is rare to be find (Ronald J. Erskine, 2016a). 2.1  Escherichia coli Escherichia coli is no longer bizarre in our communities. This Enterobacteriaceae is a Gram-negative bacteria. It cannot sporulate and can be found commonly in the intestine. This bacteria have a characteristic of glucose fermenting, acid producing, nitrate reducing and oxidase negative. It also have facultative anaerobic characteristic which can grow easily at 37 °C. Physically, E. coli is small in size, approximately 1-2  µ x 30-30  µ, with flagella. It has no capsule and also no mucus layer. There are several antigen structures that play an important role for the identification of this bacteria, which are K antigen, H antigens and O antigen. K antigen can be found in the capsules, a compound in the group of polysaccharide. H antigen can be found in flagella and O antigen can be found in the cells or the body as it is a substance in the group of lipopolysaccharide and polysaccharide (Wiwanitkit, 2011). Despite the fact that most of E. coli are harmless and beneficial, there are species that have the ability to cause disease, and they are divided into groups according to the mechanism of pathogenesis. The groups are enterohemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC) and enteroaggregative Escherichia coli (EAEC). EHEC is a group that can cause haemorrhagic colitis and can end up with a specific complication called uremia haemolytic syndrome. EIEC or also known as Shiga toxin-producing E. coli (STEC) causes diarrhea. ETEC is a major cause of travelers diarrhea and diarrhea in group of infants in developing countries. E. coli toxic products from the gut causes problems to the patients body. EPEC is a major cause of diarrhea in infants and EAEC is a new pathogen (Wiwanitkit, 2011). 2.2 Mastitis Mastitis is the inflammation of the mammary gland and udder tissue, and is a major endemic disease of dairy cattle. It commonly occurs as an immune response to bacterial invasion of the teat canal by variety of bacterial sources present on the farm, and can also occur as a result of chemical, mechanical or thermal injury to the cows udder (AHDB Dairy, 2017). 3.0 MATERIALS AND METHODS 3.1 Samples collection This study is conducted in laboratory of Faculty of Veterinary Medicine in University Malaysia Kelantan. The bacteria sample, which is Escherichia coli is isolated from the stock samples of the laboratory. There are three samples, which are EY1, E27 and E31. 3.2 Samples preparation The samples are cultured on the nutrient agar for 18 to 24 hours at 37 °C. 3.3 Isolation and identification of Escherichia coli The bacterial colony on the nutrient agar is then cultured on the eosin methylene blue (EMB) agar and gram staining is done. 3.4 Minimum Inhibitory Concentration Minimum inhibitory concentration (MIC) are determined using the broth micro dilution method. A range concentrations of antimicrobial is prepared in a 96 well microplate, followed by inoculation of bacteria culture to yield approximately 5105 cfu/ml in a 250ÃŽÂ ¼ final volume. The plate is then incubated in 37 °C for 18 hours. The lowest concentration of antimicrobial that inhibit bacterial growth is taken as MIC value. 3.5 Minimum Bactericidal Concentration The dilution representing the MIC are aliquot volume of the broth and spot onto Nutrient agar. The agar is then incubated 18 hours with 37 °C. No growth indicate that antibiotic is bactericidal at that concentration. If there is present growth indicative that antibiotic work as antibiotic only particularly on the dilution. 3.6 Time Kill Assay Tube containing designated concentration of antimicrobial will be inoculated with 105 cfu/ml of bacteria in Nutrient agar, followed by incubation at 37 °C, in an incubator shaker at 200 rpm. At 0, 1, 4, 24 hour post inoculation, 50 µ aliquots will be taken out, serially diluted in saline and plated on Nutrient agar. Plates will be incubated for 18 hours at 37 °C followed by colony counting. An antimicrobial is considered bactericidal if there is †°Ã‚ ¥3-log decrease in cfu/ml of bacteria counts, as compared to the growth control.      Ã‚   4.0 RESULTS 4.1 Isolation and identification of Escherichia coli Colony of E. coli on EMB agar is small to medium circle in size and shape, with dark green and glossy, raised and polish appearance. 4.4 Time Kill Assay SAMPLE EY1 (x103) T0 T1 T4 T24 MEDIA CONTROL 110 120 135 150 1X MIC 42 11 21 110 2X MIC 26 42 26 0 5X MIC 20 12 11 0 15X MIC 13 13 1 0 MILK CONTROL 123 135 141 156 1X MIC 46 35 173 110 2X MIC 46 26 11 100 5X MIC 32 14 8 0 15X MIC 37 13 4 0 Table 1 : Time kill Assay Results for Sample EY1 in x103 SAMPLE EY1 (x106) T0 T1 T4 T24 MEDIA CONTROL 5.5 6.0 6.75 7.5 1X MIC 2.1 0.55 1.05 5.5 2X MIC 1.3 2.1 1.3 0 5X MIC 1.0 0.6 0.55 0 15X MIC 0.65 0.65 0.05 0 MILK CONTROL 6.15 6.75 7.05 7.8 1X MIC 2.3 1.75 8.65 5.5 2X MIC 2.3 1.3 0.55 5.0 5X MIC 1.6 0.7 0.4 0 15X MIC 1.85 0.65 0.2 0 Table 2 : Time kill Assay Results for Sample EY1 in x106 Figure 3 : Time-kill curve for E. coli (EY1) against Tetracycline on media and milk SAMPLE E27 (x103) T0 T1 T4 T24 MEDIA CONTROL 113 126 141 154 1X MIC 64 29 49 111 2X MIC 58 35 18 10 5X MIC 42 22 2 0 15X MIC 30 11 1 0 MILK CONTROL 127 139 145 161 1X MIC 48 36 180 100 2X MIC 58 52 30 111 5X MIC 41 22 18 5 15X MIC 47 22 20 5 Table 3 : Time kill Assay Results for Sample E27 in x103 SAMPLE E27 (x106) T0 T1 T4 T24 MEDIA CONTROL 5.65 6.3 7.05 7.7 1X MIC 3.2 1.45 2.45 5.55 2X MIC 2.9 1.75 0.9 0.5 5X MIC 2.1 1.1 0.1 0 15X MIC 1.5 0.55 0.05 0 MILK CONTROL 6.35 6.95 7.25 8.05 1X MIC 2.4 1.8 9.0 5.0 2X MIC 2.9 2.6 1.5 5.55 5X MIC 2.05 1.1 0.9 0.25 15X MIC 2.35 1.1 1.0 0.25 Table 4 : Time kill Assay Results for Sample E27 in x106 Figure 4 : Time-kill curve for E. coli (E27) against Tetracycline on media and milk SAMPLE E31 (x103) T0 T1 T4 T24 MEDIA CONTROL 115 121 137 145 1X MIC 59 41 39 114 2X MIC 57 37 27 43 5X MIC 55 39 23 0 15X MIC 24 33 7 0 MILK CONTROL 121 140 149 165 1X MIC 56 40 180 108 2X MIC 71 50 45 116 5X MIC 51 26 12 102 15X MIC 58 25 13 0 Table 5 : Time kill Assay Results for Sample E31 in x103   SAMPLE E31 (x106) T0 T1 T4 T24 MEDIA CONTROL 5.75 6.05 6.85 7.25 1X MIC 2.95 2.05 1.95 5.7 2X MIC 2.85 1.85 1.35 2.15 5X MIC 2.75 1.95 1.15 0 15X MIC 1.2 1.65 0.35 0 MILK CONTROL 6.05 7.0 7.45 8.25 1X MIC 2.8 2.0 9.0 5.4 2X MIC 3.55 2.5 2.25 5.8 5X MIC 2.55 1.3 0.6 5.1 15X MIC 2.9 1.25 0.65 0 Table 6 : Time kill Assay Results for Sample E31 in x106       Figure 5 : Time-kill curve for E. coli (E31) against Tetracycline on media and milk